General Cell Based Assays
Cayman Chemical Cell-Based Assay Buffer Tablet, 2 ea
Cell-Based Assay Buffer Tablet has been formulated and tested to work with Cayman’s Assay Kits. Detailed instructions for its use are contained in the respective Cayman Assay Kit booklet.
Meso Scale Discovery R-PLEX Rat IL-2 ASY
The R-PLEX Rat IL-2 Assay Kit from Meso Scale Discovery measures rat interleukin 2 (IL-2) in various biological fluids using MSD s electrochemiluminescent detection technology It offers high sensitivity a wide dynamic range and low sample volume requirements The kit includes a 96-well plate with capture antibodies detection antibodies calibrators and necessary reagents Results are available in 3 5 hours using an MSD instrument This assay quantitatively measures rat IL-2 crucial for the immune system
Eurofins Discoverx AssayComplete Cell Plating 21 Reagent, 500. (93-0563R21B)
AssayComplete Cell Plating 21 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 500 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter eXpress KDR Activity Assay, 2-Plate Kit. (93-0903E3CP16M)
The PathHunter KDR Activity eXpress kit provides an easy-to-use cell based assay to study small molecule drug candidates targeting RTKs. This kit assesses RTK activation via detection of resulting KDR phosphorylation-induced SH2 domain protein recruitment. This product uses EFC technology. The cell line overexpresses ProLink-tagged fusion of the KDR kinase domain with transmembrane and extracellular ligand binding domain of EGFR; and also an EA-tagged SH2 domain protein. Upon ligand induced receptor activation, cross-phosphorylation of the receptor leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Bioassay Kit ED Detection Kit, 50 (1,000 dp, 96-well). (93-1043)
The PathHunter Bioassay ED Detection Kit is a detection kit intended for use with Eurofins DiscoverX's PathHunter Bioassay cells overexpressing ED (ProLabel/PK) but not containing any EA. This kit contains lysis buffer, exogenous EA, and EFC substrate to allow for detection of levels of ED in the assayed cells. This kit contains all components required to assay 10 plates (96-well). For detailed product information, User manual and COA, visit www.discoverx.com/products/reagents/detection-kit-93-1043.
Eurofins Discoverx PathHunter Mouse S1P1 (EDG1) β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Mouse S1P1 (EDG1) β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure EDG1 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human THR beta Nuclear Receptor Protein Interaction Cell-Based Assay Kit (CHO-K1)
The PathHunter Human THR beta Nuclear Receptor Stable Cell Line Protein Interaction Assay (CHO-K1) contains a stable clonal cell line used to measure activated THRβ nuclear hormone receptor interaction with SRCPs. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter eXpress ADCYAP1R1 Total Internalization Assay, 10-plate Kit. (93-0885E3CP0L)
The PathHunter ADCYAP1R1 Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of ADCYAP1R1 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged ADCYAP1R1 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the ADCYAP1R1 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical 8-propylthio NAD sodium sal
A derivative of NAD+; has been used in the screening of analog-sensitive PARP1 gatekeeper mutations
Eurofins Discoverx cAMP Hunter eXpress MCHR1 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0055E2CP2M)
The cAMP Hunter MCHR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. melanin concentrating hormone) based activation of MCHR1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type MCHR1, a naturally Gi/Go coupled GPCR. Upon activation of MCHR1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical Methyltransferase Assay AdoHcy Positive Control, 1 ea
Methyltransferase Assay AdoHcy Positive Control, 1 ea
Eurofins Discoverx PathHunter eXpress TGFBR1/ACVR2 Dimerization Assay, 10pl Kit. (93-1012E3CP5L)
The PathHunter TGFBR1/ACVR2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. GDF-11) induced dimerization of TGFBR1/ACVR2 receptors. This product uses EFC technology. The cell line included overexpresses TGFBR1 target protein fused to PK alongside ACVR2 target protein fused to EA. Upon ligand-induced dimerization of TGFBR1/ACVR2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery U-PLEX Human DPPIV ASY
The Meso Scale Discovery U-PLEX Human DPP4 Assay is a multiplex immunoassay panel that measures Dipeptidyl Peptidase 4 (DPP4) in human serum plasma and cell culture supernatants This assay utilizes MSD s electrochemiluminescent detection technology and is designed for use on MSD s QuickPlex SQ 120 instrument The assay has high sensitivity and wide dynamic range It requires a low sample volume of 50 L The total assay time is approximately 3 5 hours This product provides a fast simple and robust way to measure DPP4 levels across multiple samples
Eurofins Discoverx AssayComplete Cell Plating 3 Reagent, 500. (93-0563R3B)
AssayComplete Cell Plating 3 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 500 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter Human AR Nuclear Translocation Cell-Based Assay Kit (HEK 293)
The PathHunter Human AR Stable Cell Line Nuclear Translocation Assay (HEK 293) contains a stable clonal cell line used to measure AR nuclear hormone receptor activation via nuclear translocation. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.