General Cell Based Assays
Molecular Devices FLIPR Calcium 4 Kit, No-Wash, Fluorescence, 10 Plates
Molecular Devices FLIPR Calcium 4 Assay Kit Explorer provides a no-wash, fluorescence-based method for detecting intracellular calcium changes with improved signal-to-noise using a novel masking dye.
- Designed for no-wash fluorescence calcium assays.
- Includes enough reagent for 10 plates in the Explorer configuration.
- Optimized for small calcium responses with reduced background.
- Compatible with 96-well, 384-well, and 1536-well plate formats.
- Supplied as reagent vials and a dilution/assay buffer to prepare plates.
Cayman Chemical ThIol DetectIon asy KIt 480Wel
The detection and measurement of free thiols (i.e., free cysteine, glutathione, and cysteine residues on proteins) is one of the essential tasks for investigating biological processes and events in many biological systems. Cayman’s Thiol Detection provides a simple, reproducible, and sensitive tool for assaying free thiol content in samples (i.e., plasma, serum, tissue homogenates, cell lysates, and urine). The assay utilizes a proprietary fluorometric detector that reacts with thiol groups to emit a strong fluorescent signal which can be detected using excitation wavelengths between 380-390 nm and emission wavelengths between 510-520 nm.
Cayman Chemical NonIdet P40 asy Reagent1 0.5mL
An inhibitor of HRV 3C protease inhibits HRV 3C protease-induced proteolysis of HRV-14 precursor polyproteins in HRV-14-infected H1HeLa cells at 2 UM inhibits replication of 49 serotypes of HRV in HRV-infected H1HeLa cells (mean EC50 0.023 UM) inhibits replication of coxsackievirus 21 coxsackievirus B3 echovirus type 11 and enterovirus 70 in virus-infected MRC-5 cells (EC50s 0.147 0.183 0.014 and 0.007 UM respectively) decreases the number of HRV-2 PFUs in and IL-8 and IL-6 secretion by HRV-2-infected BEAS-2B cells at 10 UM inhibits enterovirus 71-induced limb paralysis and decreases in survival as well as reduces intestinal lung brain stem and cardiac muscle viral loads in enterovirus-71-infected neonatal mice at 0.1 mg/kg per day
AdipoGen Isthmin-1 human ELISA Kit
Colorimetric Sandwich ELISA Assay. Detects human Isthmin-1 in plasma and cell culture supernatant. It detects also mouse Isthmin-1 very conserved. Range 0.625 to 40ng/ml. Sensitivity 0.4 ng/ml. Samples Cell Culture Supernatant, Plasma. Isthmin-1 ISM1 was first identified as a gene expressed in the Xenopus midbrain-hindbrain organizer called the isthmus, with a proposed role during early brain development. Isthmin-1 encodes a predicted ~50-kDa protein containing a signal peptide, a thrombospondin domain and an adhesion-associated domain. Isthmin-1 is important for embryonic and postnatal development. Growing evidence has shown that aberrant expression of Isthmin-1 can also affect the biological behavior of cancer. The Ism1 gene is conserved in mice and humans. A recent study showed that Isthmin-1 is an adipokine that induces glucose uptake in human and mouse adipocytes.
Eurofins Discoverx cAMP Hunter Follitropin Alfa Bioassay Kit, 2pl. (95-0119Y2-00103)
The cAMP Hunter Follitropin Alfa Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses Follitropin Alfa based activation of FSHR GPCR activity via detection of levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type FSHR, a naturally Gs coupled GPCR. Upon activation of FSHR, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Medchemexpress LLC cGAS Human His-SUM 10ug
Recombinant human cyclic GMP-AMP synthase (cGAS) fragment G161-F522, expressed in E. coli and supplied lyophilized with an N-terminal 6xHis-SUMO tag. The protein is purified to >90% and provided with storage and reconstitution guidance to preserve activity for biochemical and structural applications.
- Recombinant human cGAS fragment G161-F522 with N-terminal His-SUMO tag.
- Expressed in E. coli and purified to greater than 90% purity.
- Lyophilized formulation for improved stability and convenient storage.
- Buffer contains trehalose to protect protein during lyophilization.
- Suitable for biochemical assays, enzymatic activity, and structural studies.
- Includes storage and reconstitution guidance to maintain activity.
Eurofins Discoverx PathHunter eXpress P2RY11 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0281E2CP2L)
The PathHunter P2RY11 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. ATP) based activation of P2RY11 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged P2RY11 and EA-tagged β-Arrestin-2. Activation of P2RY11 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Indigo Biosciences Human Granulocyte Colony-Stimulating Factor Receptor (G-CSFR) All-inclusive Cell-Based Luciferase Reporter Assay Kit, 96-well assay plate format
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO’s Human Granulocyte Colony-Stimulating Factor Receptor reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against G-CSFR. Kit includes cryopreserved reporter cells engineered to provide constitutive expression of the receptor, optimized media for use during cell thawing and in diluting test samples, reference agonist, Luciferase Detection Reagent, cell culture-ready assay plate, and detailed assay protocol. INDIGO’s unique Cryomite™ process provides exceptional cell viability post-thaw and INDIGO’s strict QA process means consistent results lot to lot (product quality data available upon request). This kit allows researchers to achieve superior sensitivity, clear, reproducible results, and eliminate weeks of cell culture work.
Indigo Biosciences Human Adrenoceptor Alpha 1B (ADRA1B) All-inclusive Cell-Based Luciferase Reporter Assay Kit, 96-well assay plate format
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO’s Human Adrenoceptor Alpha 1B reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against ADRA1B. Kit includes cryopreserved reporter cells engineered to provide constitutive expression of the receptor, optimized media for use during cell thawing and in diluting test samples, reference agonist, Luciferase Detection Reagent, cell culture-ready assay plate, and detailed assay protocol. INDIGO’s unique Cryomite™ process provides exceptional cell viability post-thaw and INDIGO’s strict QA process means consistent results lot to lot (product quality data available upon request). This kit allows researchers to achieve superior sensitivity, clear, reproducible results, and eliminate weeks of cell culture work.
Eurofins Discoverx PathHunter eXpress C5AR1 Activated GPCR Internalization Assay, 2pl Kit. (93-0777E3CP5M)
The PathHunter C5AR1 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of C5AR1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged C5AR1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting C5AR1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.